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Real-time PCR vs.
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 PCR BOOKS: A list of useful and available books on Polymerase Chain Reaction Technique since 1990 to 2002 as well as PCR Books that will be published in 2003. This section contain 74 links with Title, Publication year, and Edition number to vendors of these books as well as additional information. PCR Books are sorted by publishing year, and then alphabetically. Check out this section at http://www.pcrlinks.com/resources/books1.htm
DNA Amplification: Current Technologies and Applicationshttp://www.horizonpress.com/hsp/abs/absdna.html
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to
real-time PCR Literature in WWW:
 |
Real-Time PCR: An Essential Guide Publisher:
Horizon Bioscience |
- PCR and real-time PCR (in general):
Improved mRNA Quantitation in LightCycler RT-PCR
Int Arch Allergy Immunol 2003;130:82–86
Bernard PS, Wittwer CT.
(2000)
Homogeneous
amplification and variant detection by fluorescent hybridization probes.
Clin Chem. 2000
Feb;46(2):147-8.
Belgrader P, Young S, Yuan B,
Primeau M, Christel LA, Pourahmadi F, Northrup MA. (2001)
A battery-powered
notebook thermal cycler for rapid multiplex
real-time PCR analysis.
Anal Chem. 2001 Jan
15;73(2):286-9.
Boulay JL, Reuter J, Ritschard
R, Terracciano L, Herrmann R, Rochlitz C. (1999)
Gene dosage by
quantitative real-time PCR.
Biotechniques. 1999
Aug;27(2):228-30, 232.
Brownie J, Shawcross S, Theaker
J, Whitcombe D, Ferrie R, Newton C, Little S. (1997)
The elimination of
primer-dimer accumulation in PCR.
Nucleic Acids Res.
1997 Aug 15;25(16):3235-3241
Burkardt HJ (2000)
Standardization and
quality control of PCR analyses
Clin Chem Lab
Med 2000 Feb;38(2):87-91
Bustin SA. (2000)
Absolute
quantification of mRNA using real-time reverse transcription polymerase
chain reaction assays.
J Mol Endocrinol.
2000 Oct;25(2):169-93. Review.
Bustin SA. (2002)
Quantification of
mRNA using real-time reverse transcription PCR: trends and problems
J Mol Endocrinol.
2002 29: 23-29 Review.
Chandler DP, Wagnon CA, Bolton
H Jr. (1998)
Reverse
transcriptase (RT) inhibition of PCR at low concentrations
of template and its implications
for quantitative
RT-PCR.
Appl Environ
Microbiol. 1998 Feb;64(2):669-77.
de Silva D, Wittwer CT.
(2000)
Monitoring
hybridization during polymerase chain reaction.
J Chromatogr B
Biomed Sci Appl. 2000 Apr 28;741(1):3-13. Review.
de Kok, J.B., Hendriks, J.C.,
van Solinge, W.W., Willems, H.L., Mensink, E.J., and Swinkels,
D.W. (1998)
Use of real-time
quantitative PCR to compare DNA isolation methods. Clin.Chem.
44(10):2201-2204, 1998.
DNA / RNA real-time
quantitative PCR (????)
PE Biosystems
Elenitoba-Johnson KS, Bohling
SD, Wittwer CT, King TC. (2001)
Multiplex PCR by
multicolor fluorimetry and fluorescence melting curve analysis.
Nat Med. 2001
Feb;7(2):249-53.
Enard et al. (2002)
Intra- and
Interspecific Variation in Primate Gene Expression Patterns
Science 296 (5566):
340-343
Ferré, F (1992)
Quantitative or
semi-quantitative PCR: Reality versus Myth.
PCR Methods and
Applications 2, 1-9.
Fitzgerald DA,
(2000)
The Scientist 14
(23):31, Nov. 27, 2000
Freeman, WM, Walker, SJ, and
Vrana, KE (1999)
Quantitative RT-PCR:
pitfalls and potential. Biotechniques 26, 112-122.
Giulietti A, Overbergh L,
Valckx D, Decallonne B, Bouillon R, Mathieu C. (2001)
An overview of
real-time quantitative PCR: applications to quantify cytokine gene
expression.
Methods 2001
Dec;25(4):386-401
Gibson, UE, Heid, CA, and
Williams, PM (1996)
A novel method for
real time quantitative RT-PCR. Genome Res 6, 1095-1001.
Ginzinger DG. (2002)
Gene quantification
using real-time quantitative PCR. An emerging technology hits the
mainstream
Exp Hematol
2002 Jun;30(6):503-12
Higuchi, R, Fockler, C,
Dollinger,
G, and Watson, R (1993)
Kinetic PCR
analysis: real-time monitoring of DNA amplification reactions.
Biotechnology 11,
1026-1030.
Jung R, Soondrum K, Neumaier M.
(2000)
Quantitative
PCR.
Clin Chem Lab Med.
2000 Sep;38(9):833-6. Review.
Kainz P. (2000)
The PCR plateau
phase - towards an understanding of its
limitations.
Biochim Biophys
Acta 2000 Nov 15;1494(1-2):23-7
Klein D. (2002)
Quantification using
real-time PCR technology: Applications and limitations.
Trends Mol Med
2002 Jun;8(6):257-60
Lekanne Deprez RH, Fijnvandraat
AC, Ruijter JM, Moorman AF. (2002)
Sensitivity and
accuracy of quantitative real-time polymerase chain reaction using SYBR
green I depends on cDNA synthesis conditions.
Anal Biochem. 2002
307(1): 63-69.
Lie, Y. S. and C. J.,
Petropoulos. (1998)
"Advances in
quantitative PCR technology: 5' nuclease assays."
Curr Opin Biotechnol
9 (1998): 43-48.
Liss B.
Improved
quantitative real-time RT-PCR for expression profiling of individual
cells (2002)
Nucleic Acids
Res 2002 Sep 1;30(17):e89
Liu W & Saint DA.
(2002)
A new quantitative
method of real time reverse transcription polymerase chain
reaction
assay based on
simulation of polymerase chain reaction kinetics.
Anal Biochem.
2002 302(1): 52-59.
Liu W & Saint DA
(2002)
Validation of a
quantitative method for real time PCR kinetics.
Biochem Biophys Res
Commun. 2002 294(2): 347-353
Mackay IM, Arden KE, Nitsche A.
(2002)
Real-time PCR in virology.
Nucleic Acids Res.
2002 Mar 15;30(6):1292-305. Review.
Mannhalter, C, Koizar, D, and
Mitterbauer, G (2000)
Evaluation of RNA
isolation methods and reference genes for RT-PCR analyses of
rare target RNA.
Clin Chem Lab Med 38, 171-177.
Morrison TB, Weis JJ, Wittwer
CT. (1998)
Quantification of
low-copy transcripts by continuous SYBR Green I monitoring during
amplification.
Biotechniques. 1998
Jun;24(6):954-8, 960, 962.
Nath K, Sarosy JW, Hahn J,
Di Como CJ. (2000)
Effects of ethidium
bromide and SYBR Green I on different polymerase chain reaction systems.
J Biochem Biophys
Methods. 2000 Jan 3;42(1-2):15-29.
Orlando, C, Pinzani, P, and
Pazzagli, M (1998)
Developments in
quantitative PCR. Clin Chem Lab Med 36,
255-269.
Peccoud J &
Jacob C. (1998)
Gene Quantification
(eds. Francois Ferre)
Statistical
estimations of PCR amplification rates.
Ramakers C, Ruijter JM, Deprez
RH, Moorman AF. (2003)
Assumption-free
analysis of quantitative real-time PCR data
Neurosci Lett
2003 Mar 13;339(1): 62-66
Schmittgen
TD. (2001)
Real-time
quantitative PCR. Methods. 2001 Dec;25(4): 383-385.
Schmittgen TD, Zakrajsek BA,
Mills AG, Gorn V, Singer MJ, Reed MW. (2000)
Quantitative reverse
transcription-polymerase chain reaction
to study mRNA decay: comparison of endpoint and real-time methods.
Anal Biochem. 2000
Oct 15;285(2):194-204.
Sequence detection systems
quantitative assay design and optimization. (????)
PE Biosystems
Souazé, F,
Ntodou-Thomé, A, Tran, CY, Rostene, W, and Forgez, P (1996)
Quantitative
RT-PCR: Limits and accuracy. BioTechniques 21, 280-285.
Svanvik N., G. Westman, W.
Dongyuan & M. Kubista. (2000)
Light-up Probes
Thiazole Orange Conjugated PNA for Detection of Nucleic Acid in
Homogeneous Solution.
Anal. Biochem. 281,
26-35 (2000).
Svanvik N., A. Stålberg,
U. Sehlstedt, R. Sjöback & M. Kubista. (2000)
Detection of PCR
Products in Real-time Using Light-up Probes.
Anal. Biochem. 287,
179-182 (2000).
Svanvik N., J. Nygren, G.
Westman & M. Kubista. (2001)
Free-probe
Fluorescence of Light-up probes.
J. Amer. Chem. Soc.,
123, 803-809 (2001).
Ales Tichopad, Anamarija
Dzidic, Michael W. Pfaffl (2002)
Improving
quantitative real-time RT-PCR reproducibility
by boosting
primer-linked amplification efficiency
Biotechnology
Letters 24: 2053-2056 (2002)
Ales Tichopad, Michael Dilger,
Gerhard Schwarz & Michael W. Pfaffl
(2003)
Standardized
determination of real-time PCR effciency from a single reaction set-up.
Nucleic Aids
Research 31(20): e122 (2003)
Ales Tichopad, Andrea Didier,
Michael W. Pfaffl (2003)
Inhibition
of real-time RT–PCR quantification due to tissue-specific contaminants
Molecular and Cellular Probes (2003)
Vandesompele J, De Paepe A,
Speleman F. (2002)
Elimination of
Primer-Dimer Artifacts and Genomic Coamplification Using a
Two-Step SYBR Green
I Real-Time RT-PCR. Anal Biochem. 2002 Apr 1;303(1):95-98
Nigel J. Walker (2002)
A Technique Whose
Time Has Come
SCIENCE VOL 296 19
APRIL 2002
Teo IA, Choi JW, Morlese J,
Taylor G, Shaunak S. (2002)
LightCycler qPCR
optimisation for low copy number target DNA
J Immunol
Methods 2002 Dec 1;270(1):119-33
Wang, AM, Doyle, MV, and Mark,
DF (1989)
Quantitation of mRNA
by the polymerase chain reaction. PNAS 86, 9717-9721.
Weis JH, Tan SS, Martin BK,
Wittwer CT. (1992)
Detection of rare
mRNAs via quantitative RT-PCR.
Trends Genet. 1992
Aug;8(8):263-4.
Whitcombe, D., Theaker J., Guy,
S.P., Brown, T., Little, S. (1999)
Detection of PCR
products using self-probing amplicons and flourescence.
Nature 17, 804-807
Wilson IG. (1997)
Inhibition and
facilitation of nucleic acid amplification.
Appl Environ
Microbiol. 1997 Oct;63(10):3741-51. Review.
Wittwer CT, Herrmann MG, Gundry
CN, Elenitoba-Johnson KS. (2001)
Real-Time Multiplex
PCR Assays.
Methods. 2001
Dec;25(4):430-442.
Wittwer CT, Ririe KM, Andrew
RV, David DA, Gundry RA, Balis UJ. (1997)
The LightCycler: a
microvolume multisample fluorimeter with rapid temperature control.
Biotechniques. 1997
Jan;22(1):176-81.
Wittwer CT, Herrmann MG, Moss
AA, Rasmussen RP. (1997)
Continuous
fluorescence monitoring of rapid cycle DNA amplification.
Biotechniques. 1997
Jan;22(1):130-1, 134-8.
Wittwer CT, Garling
DJ. (1991)
Rapid cycle DNA
amplification: time and temperature optimization.
Biotechniques. 1991
Jan;10(1):76-83.
Wittwer CT, Fillmore GC,
Hillyard DR. (1989)
Automated polymerase chain reaction in capillary tubes with
hot air.
Nucleic Acids Res.
1989 Jun 12;17(11):4353-7.
Wong, L, Pearson, H, Fletcher,
A, Marquis, CP, and Mahler, S (1998)
Comparison of the
efficiency of M-MuLV reverse transcriptase, Rnase H—M-MuLV reverse
transcriptase
and AMV reverse
transcriptase for the amplification of
human immunglobulin genes.
Biotechnology
Techniques 12, 485-489.
Gene expression profiling using a novel method: amplified differential gene expression (ADGE)
Nucleic Acids Res. 2001; 29: e46
| Publications
by Roche Molecular Biochemicals
Link -> LightCycler Literature Request Form Link -> References & Literature: LightCycler Articles Published in Biochemica |
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|
 Pfaffl, MW (2001) Development and validation of an externally standardised quantitative Insulin like growth factor-1 (IGF-1) RT-PCR using LightCycler SYBR ® Green I technology. In: Meuer, S, Wittwer, C, Nakagawara, K, eds. Rapid Cycle Real-time PCR, Methods and Applications Springer Press, Heidelberg, ISBN 3-540-66736-9 |
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| LightCycler Relative Quantification Software, Version 1.0 (2001) |
|
| Technical
Note No. LC 1/1999 How to Reduce Primer Dimers in a LightCycler PCR |
|
| Technical
Note No. LC 4/1999 Adaptation Protocol for Sequence-Independent Detection of RNA with SYBR Green I |
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| Technical
Note No. LC 2/1999 Adaptation Protocol for Sequence-Independent Detection of DNA with SYBR Green I |
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| Technical
Note No. LC 9/2000 Optimization Strategy |
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| Technical
Note No. LC 10/2000 Overview of LightCycler Quantification Method |
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| Technical
Note No. LC 11/2000 Absolute Quantification with External Standards |
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| Technical
Note No. LC 12/2000 Absolute Quantification with External Standards and an Internal Controls |
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| Technical
Note No. LC 13/2001 Relative Quantification |
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| Technical Note No. LC 15/2002 Selection of Housekeeping Genes |
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|
Technical Support: Literature
Series on Technical Aspects of the LightCycler System |
|
| Soong, R,
Ruschoff, J, and Tabiti, K (2000) Detection of colorectal micrometastasis by quantitative RT-PCR of cytokeratin 20 mRNA. (Roche Molecular Biochemicals Internal Publication) |
|
| LightCycler
Softwares http://www.lightcycler-online.com/lc_sys/soft_ind.htm |
|
| LightCycler
Manuals http://www.lightcycler-online.com/lc_support/lc_tech_man.htm |
|
- absolute quantification:
Expression of estrogen and progesterone receptors in the
bovine ovary during estrous cycle and pregnancy.
Endocrine 2002 Apr;17(3):207-214
Bustin SA.
Absolute quantification of mRNA using real-time reverse
transcription (2000)
polymerase chain
reaction assays.
J Mol Endocrinol.
2000 Oct;25(2):169-93. Review.
A method for the rapid construction of cRNA standard curves in quantitative real-time RT-PCR.
Mol Cell Probes 2002 Apr;16(2): 99-110
Georgiev I P, T M Georgieva, M
Pfaffl1, H M Hammon and J W Blum (2003)
Insulin-like growth
factor and insulin receptors in intestinal
mucosa of neonatal calves.
Journal of
Endocrinology, 176: 121-132
Inderwies T., M. W. Pfaffl, H. H. D.
Meyer, J. W. Blum & R. M. Bruckmaier (2003)
Detection
and quantification of mRNA-expression of alpha- and beta-adrenergic
receptor subtypes
in
the mammary gland of dairy cows.
Domestic
Animal Endocrinology (24): 123-135
Milking characteristics and their relation to adrenergic receptor mRNA expression and ligand binding in the mammary gland of dairy cows.
Domestic Animal Endocrinology 2003 (25): 275-286
Ke LD, Chen Z, Yung WK. (2000)
A reliability test
of standard-based quantitative PCR: exogenous vs endogenous standards.
Mol Cell Probes.
2000 Apr;14(2):127-135.
Reidun Ovstebo,* Kari Bente
Foss Haug,
Knut Lande, and Peter Kierulf (2003)
PCR-based
Calibration Curves for Studies of Quantitative Gene Expression in Human
Monocytes: Development and Evaluation.
Clinical Chemistry
49:3 425–432 (2003)
LightCycler Software ®,
Version 3.5 (2001)
Roche Molecular
Biochemicals.
Development and validation of an externally standardised quantitative Insulin like growth factor-1 (IGF-1) RT-PCR using LightCycler SYBR ® Green I technology.
Pfaffl, MW (2001)
Development and
validation of an externally standardised quantitative Insulin like
growth factor-1 (IGF-1) RT-PCR using LightCycler SYBR ® Green I
technology.
Biochemica
3/2000 - ROCHE Diagnostics
M. W. Pfaffl & M.
Hageleit (2001)
Validities of mRNA
quantification using recombinant RNA
and recombinant DNA external calibration
curves in real-time
RT-PCR. Biotechnology Letters (2001) 23, 275-282
Pfaffl MW, Lange IG,
Daxenberger A,
Meyer HH. (2001)
Tissue-specific
expression pattern of estrogen receptors (ER): quantification of ER
alpha and ER
beta mRNA with
real-time RT-PCR. APMIS. 2001 May;109(5):345-55.
The gastrointestinal tract as target of steroid hormone action: Quantification of steroid receptor mRNA expression (AR, ER-alpha, ER-beta and PR) in 10 bovine gastrointestinal tract compartments by kinetic RT-PCR.
Journal of Steroid Biochemistry & Molecular Biology (2003) 84 (2-3): 159-166
M.W. Pfaffl, T. Mircheva
Georgieva, I. Penchev Georgiev, E. Ontsouka, M. Hageleit & J. W.
Blum (2002)
Real-time
RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1
receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone
receptor, IGF-binding proteins 1, 2 and 3 in the bovine species.
Domest Anim
Endocrinol, 22(2): 91-102.
M.W. Pfaffl, A. Daxenberger, M.
Hageleit & H.H.D. Meyer (2002)
Effects of synthetic
progestagens on the mRNA Expression of Androgen Receptor, Progesterone
Receptor, Estrogen Receptor a and b, Insulin-like Growth Factor (IGF)-1
and IGF-1 receptor in Heifer tissues
J Vet Med A 49: 57-64
M. W. Pfaffl, S. L. Wittmann,
H. H.
D. Meyer & R. M. Bruckmaier (2003)
Gene Expression of
Immunologically Important Factors
in Blood Cells, Milk
Cells and Mammary Tissue of Cows
Jornal of Dairy
Science 86(2): 538-545
Ramakers C, Ruijter JM, Deprez
RH, Moorman AF. (2003)
Assumption-free
analysis of quantitative real-time PCR data
Neurosci Lett
2003 Mar 13;339(1): 62-66
S. Sauter, E. Ontsouka, B. Roffler, Y. Zbinden, C. Philipona, M. W. Pfaffl, B. H. Breier, J. W. Blum & H. M. Hammon (2003)
Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves
Am J Physiol Endocrinol Metab 285: E252-261
Schams D, Kohlenberg S, Amselgruber W, Berisha B, Pfaffl MW, Sinowatz F. (2003)
Expression and localisation of oestrogen and progesterone receptors in the bovine mammary
gland during development, function and involution
J Endocrinol 2003 May;177(2): 305-317
Ales Tichopad, Michael W.
Pfaffl, Andrea Didier (2003)
Tissue-specific
expression pattern of bovine prion gene:
quantification using
real-time RT-PCR
Mol Cell
Probes 17: 5-10
Vandenbroucke II, Vandesompele
J, Paepe AD, Messiaen L. (2001)
Quantification of
splice variants using real-time PCR. Nucleic Acids Res. 2001 Jul
1;29(13): E68
Wittmann SL, Pfaffl M., Meyer
HHD & Bruckmaier RM (2002)
5-Lipoxygenase,
cyclo-oxygenase-2 and tumor necrosis factor alpha gene expression in
somatic milk cells
Milk Science
International, 57(2): 63-66.
- relative quantification:
Relative quantification of gene expression.
http://docs.appliedbiosystems.com/pebiodocs/04303859.pdf
A. Hartel, A. Didier, M.W.
Pfaffl, Heinrich H.D. Meyer (2003)
Characterisation of
gene expression patterns in 22RV1 cells for determination of environmental
androgenic/antiandrogenic compound
Journal of Steroid
Biochemistry & Molecular Biology (2003) 84 (2-3): 231-238
Horgan, GW, and Rouault J (2000)
Introduction to
randomisation tests, Biomathematics and
Statistics, Scotland
http://www.bioss.ac.uk/smart/unix/mrandt/slides/frames.htm
LightCycler Relative
Quantification Software, Version 1.0 (2001)
Roche Molecular
Biochemicals.
Livak KJ, Schmittgen TD.
(2001)
Analysis of Relative
Gene Expression Data Using Real-Time Quantitative PCR and the
2(- delta
deltaC(T)) Method.
Methods. 2001
Dec;25(4): 402-408.
Pfaffl MW. (2001)
A new mathematical
model for relative quantification in real-time RT-PCR.
Top NAR
Paper 2001 http://www3.oup.co.uk/jnls/list/nar/special/1/default.html#Top
Methods
Nucleic Acids Res.
2001 May 1; 29(9):E45
Michael W. Pfaffl, Graham W.
Horgan & Leo Dempfle (2002)
Relative Expression
Software Tool (REST©) for group wise comparison and statistical
analysis
of relative
expression results in real-time PCR
Nucleic Acids
Research 2002 May 1; 30(9):E36
Rasmussen, R (2001)
Quantification on
the LightCycler. In: Meuer, S, Wittwer, C, Nakagawara, K, eds.
Rapid Cycle
Real-time PCR, Methods and Applications Springer
Press, Heidelberg; page 21-34.
http://www.idahotec.com/lightcycler_u/lectures/quantification_on_lc.html
Soong R, Beyser K, Basten O,
Kalbe A, Rueschoff J, Tabiti K. (2001)
Quantitative reverse
transcription-polymerase chain reaction
detection of
cytokeratin 20 in
noncolorectal lymph nodes.
Clin Cancer Res.
2001 Nov;7(11): 3423-3429.
Anders Ståhlberg, Pierre
Åman, Börje Ridell, Petter Mostad, and Mikael Kubista (2003)
A quantitative
real-time PCR method for detection of B-Lymphocyte Monoclonality by
comparison of kappa and lambda Immunoglobulin Light Chain Expression.
Clinical Chemistry
49(1): 51-59
Technical Note No. LC 13/2001 -
Relative Quantification
- classical block RT-PCR & competitive RT-PCR:
Effects of muscle type, castration, age, and compensatory growth rate on androgen
receptor mRNA expression in bovine skeletal muscle. J Anim Sci. 2000 Mar;78(3):629-37.
Competitive quantitative
RT-PCR (11 / 1998)
by Ambion Inc.
Elsaesser, F., Pfaffl, M.W.,
Meyer, H.H.D., Serpek, B. and Sauerwein, H. (2002)
Differences in the
somatotropic axis, in blood cortisol, insulin and thyroid hormone
concentrations between two pig genotypes with markedly divergent growth
rates and the effects of growth hormone treatment.
Animal Science, 74
(3): 423-430
Malucelli A, Sauerwein H,
Pfaffl MW, Meyer HH. (1996)
Quantification of
androgen receptor mRNA in tissues by
competitive co-amplification of a
template in reverse
transcription-polymerase chain reaction.
J Steroid Biochem
Mol Biol. 1996 Aug;58(5-6):563-8.
Pfaffl MW (1997) GERMAN
Analytische Untersuchungen zum
allometrischen Muskelwachstum beim Rind: Quantifizierung der
Expression des
insulinähnlichen Wachstumsfaktors-1 und rekombinante Herstellung
des
Wachstumshormonbindungsproteins
ISBN 3-89675-198-0
· 1997, 20,5 x
14,5 cm · broschiert; 200 Seiten · 50
Abb. · 24 Tab.; 49,50 EUR
Endokrine
Regulationsmechanismen bieten zur Erklärung allometrischer
Muskelwachstumsvorgänge keine
ausreichende
Erklärung. Das individuelle Muskelwachstum unterliegt offenbar
auch einer lokalen Regulation. Als ein hier in Frage kommender Faktor
wurde IGF-1 untersucht, für dessen mRNA eine kompetitive RT-PCR
entwickelt wurde zur präzi-sen und sensitiven Quantifizierung der
IGF-1 mRNA in Rindergeweben.
Kastration und
Ernährung hatten unterschiedliche, gewebespezifische Effekte auf
die IGF-1 Expression. Zur
Untersuchung der
Regulation des Wachstumshormon-Rezeptors (GHR) und des Bindungsproteins
(GHBP), wurden mit der rekombinanten Darstellung von GHBP in E. Coli
die methodischen Voraussetzungen (Nutzung des Proteins als Antigen zur
Antikörperdarstellung und als Standardsubstanz) geschaffen. Die
Überexpression wurde optimiert, eine Reindarstellung sowie
Charakterisierung des GHBP auch in Hinblick auf seine biologische
Aktivität ist erfolgt.
Pfaffl M, Meyer HH, Sauerwein
H. (1998)
Quantification of
insulin-like growth factor-1 (IGF-1) mRNA: development and validation
of
an internally
standardised competitive reverse transcription-polymerase chain
reaction.
Exp Clin Endocrinol
Diabetes. 1998;106(6):506-13.
M. Pfaffl; F. Schwarz & H.
Sauerwein (1998)
Quantification of
the insulin like growth factor-1 (IGF-1) mRNA: Modulation of growth
intensity by feeding results in inter- and intra-tissue specific
differences of IGF-1 mRNA expression in steers
Experimental and
Clinical Endocrinology & Diabetes 106: 513-520.
Quantitative end-point RT-PCR
gene expression measurement using the Agilent 2100
(2001)
Bioanalyzer and
stndardised RT-PCR. (2001) "StaRT-PCR" technology is a
standardized,
quantitative method
used to measure gene expression at
the mRNA level in human tissues.
Raeymaekers L. (2000) Basic
principles of quantitative PCR. (2000)
Mol Biotechnol. 2000
Jun;15(2):115-22.
Sauerwein H.; Pfaffl M.;
Hagen-Mann K.; Malucelli A. & Meyer H.H.D. (1995)
Expression of
estrogen and androgen receptor in the bovine
gastrointestinal tract.
Deutsche
tierärztliche Wochenschrift 1995, 102: 164-168.
SauerweinH., A. Brandstetter,
M.W. Pfaffl, H.H.D. Meyer, E. Möstl, J. Handler & K. Arbeiter
(1998)
Uterine Androgen
Receptor mRNA Expression in Metestrous and Anestrous Bitches being
healthy or suffering from pyometra.
Deutsche
tierärztliche Wochenschrift 105: 173-208.
Wall SJ, Edwards DR. (2002)
Quantitative reverse
transcription-polymerase chain reaction (RT-PCR): a comparison of
primer-dropping, competitive, and real-time RT-PCRs
Anal Biochem
2002 Jan 15;300(2):269-73
Y. Cao, Houren Wei, Da
Banghong, Michael W. Pfaffl & Li Zhongyu (2002)
Insulin-like growth
factor-1 gene cloning and protein expression in bovine trabecular
Meshwork tissue and cells Department of Ophthalmolgy, Union Hospital of
Tongji Medical University, Wuhan, China, and *Institute of Physiology,
München Technical University, Freising-Weihenstephan, Germany
Journal of Huazhong
University of Science and Technology 22(1): 69-72.
Insulinartige Wachstumsfaktor-1- (IGF-1-)mRNA und IGF-1-Protein Expression in den Zellen des Trabekelwerks im Rinderauge
Bovine trabecular meshwork cells express insulin-like growth factor-1 (IGF-1) mRNA and protein
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