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The Reference in qPCR & dPCR - Academic & Industrial Information Platform

Founded February 2002 -- Since 22 years, the Gene Quantification platform
describes and summarises all technical aspects involved in quantitative gene expression analysis using real-timePCR (qPCR) & digital-PCR (dPCR) & Next Generation Sequencing. It presents the majority of new and innovative qPCR & dPCR applications,
chemistries, methods, data analysis algorithms, MIQE & QC strategies, cyclers, kits, dyes, analysis methods, interesting events & workshops, and services involved. Please browse our page directory with all essential keywords or the qPCR platform, a summary of commercial and academic institutions presenting their PCR related tools.
Amplify your knowledge in qPCR, dPCR and NGS!  Follow our streaming server presenting 500 recorded talks =>        

Analytik Jena left tower banner

MIQE iBook ... 5th eds

EV & Exosomes & EV RNA / Liquid Biopsy / Biomarker Discovery Transcriptional Biomarkers
Books eBooks ePub
qPCR Platform / Protocols / MethodsTranslation / ASIA portal / GQ Page Directory
eConferences & Webinars

"free access" to 500 recorded talks Gene Quantification Events 2010-2023 / Talks / Webinars
Quantification Strategy
Quantification strategies:  absolute Quantification & relative Quantification / qPCR Evolution

RT-qPCR normalisation strategies & microRNA / Genorm / BestKeeper / RefGenes
qPCR & Optimisation
real-time PCR / interesting reviews / MIQE & Apps & iBook / Primer-Recources & Algorithms
Bioinformatics & Statistics
Big Data in Biology / data analysis / Regulatory Networks data clustering / Integrated Analysis
Software Downloads

REST (main page) / REST-2009 / RDML / RefGenes / GenEx 7.0 ... FREE DOWNLOAD
RNAi -- RNA interference

siRNA / saRNA / microRNA & microRNA normalisation / mirtron / qPCR statistics
Gene Quantification Events
impressions GQ2023 / qPCR dPCR NGS 2023 / MicroGenomics 2023 / eConferences / previous Events
digital PCR  & qPCR Array
digital-PCR ... UPDATE / MIQE & dPCR / qPCR array / normalisation and analysis software
Cyclers & Robotics
real-time PCR hardware:  overview of qPCR cyclers  / qPCR robotics / page statistics
reverse transcription / mRNA transcript analysis ... UPDATE / RNA QC & RNA / DNA integrity

Dyes  & HRM dyes

detection dyes, probes, and chemistries in real-time PCR / HRM dyes / Chips & Lab-on-Chip
Liquid Biopsy & Biomarkers
Exosomes / EV & exRNA ... UPDATE / CNA / microRNA / Transcriptional Biomarkers
Molecular Physiology
CNA / HRM / CNV / microRNA / siRNA / saRNA / RNAi / digital-PCR / single-cell qPCR
qPCR Efficiency
determination of real-time qPCR efficiency by various methods ... new efficiency papers!
qPCR & dPCR applications

EVs & Exosomes / circulating nucleic acids / digital-PCR / single-cell handling / single-cell qPCR
GQ info portal

microRNA / CNV / microRNA transfer / HRM / RNAi / siRNA / saRNA / Liquid Biopsy

logo qPCR NEWS
Gene Quantification
is sponsored by

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qPCR platform participating companies:
qPCR platform participating companies in alphabetical order

button Stilla Technologies
Stilla Technologies - NIO™ DPCR, the next leap forward in digital PCR.

button Bioron 2023
BIORON – Next Generation Tools for Life Science

logo Analytik Jena
Analytik Jena offers a wide produt variety of thermal cyclers as well as real-time thermal cyclers.

button NEB New England BioLabs -- Luna® qPCR and RT-qPCR Reagents for rapid, sensitive and precise detection of RNA, DNA and cDNA targets

logo Qiagen
Qiagen - Sample to insight

logo Bio-Rad Bio-Rad Laboratories - A complete solution. A focused approach.
BioMolecular Systems - Meet Mic. Fast. Accurate. Compact.

Eurogentec logo
Eurogentec - Your one-stop-shop Real-Time PCR supplier

Biozym button
Biozym - Science is our business

logo Bioline
Bioline – The PCR Company

button Promega real-time PCR Promega - Be successful in your qPCR experiments with our robust amplification master mixes Life Technologies - Advanced gene expression services

TIB-molbiol - Our business is DNA.
LGC - Biosearch Technologies - Advancing Nucleic Acid Technologies
Quanta BioSciences
QuantaBio is dedicated to perfecting qPCR reagents that deliver superior performance you can rely on.

logo nexttec
nexttec Biotechnologie GmbH - the fastest way to get PURIFIED DNA - tested millions of time

logo BioSistemika BioSistemika - Software development partners for life sciences - Helping deliver your ideas! PrimerDesign - Driving technology, Empowering researchers

Your want to join the qPCR or eConferences platform!  Contact us via

Cooperating Institutions & Laboratories:
logo MultiD

GenEx software - GenEx offers advanced methods to analyze real-time qPCR data with simple clicks of the mouse. The methods are suitable to select and validate reference genes, classify samples, group genes, monitor time dependent processes and much more.

Prof. Stephen Bustin - Stephen Bustin is Professor of Molecular Medicine at Anglia Ruskin University at the Postgraduate Medical Institute.

Laboratory of Gene Expression
Prof. Mikael Kubista´s
laboratory of Gene Expressionis at the Institute of Biotechnology (BTU) @ Czech Academy of Science (CAS). The Institute of Biotechnology is member of BIOCEV, CzechBIO, The Technology Centre, Czech Academy of Science (CAS) BIOCEV.
is group is developing real-time PCR assays for different applications such as routine clinical diagnostic and methods for high through put measurements

GeneCore offers service in the field of single-cell and spatial transcriptomics, with 18 years experience with single-cell profiling.
At our state-of-the-art GeneCore service laboratory, they specialize in cutting-edge techniques for RNA library preparation, empowering researchers to uncover the intricate genetic mechanisms behind biological processes.
With their long lasting expertise in the RNA analysis, they offer comprehensive analysis of individual cells, enabling a deeper understanding of cellular diversity, heterogeneity and gene expression patterns.
The GeneCore laboratory is equipped with advanced spatial transcriptomic technology, allowing researchers to visualize gene expression within intact tissue samples, unraveling the spatial organization of cellular activity. The team takes pride in delivering accurate and reliable results through our meticulous RNA sequencing workflows, ensuring high-quality data for your research needs. Collaborating with leading scientists and utilizing the latest sequencing platforms, our service laboratory is committed to providing exceptional services in the field of RNA sequencing, single cell RNA sequencing, and spatial transcriptomics.

EMBL GeneCore is the in-house genomics service center at EMBL in Heidelberg, Germany, for DNA sequencing, next-generation-sequecing (NGS), S.A.G.E., microarrays, real-time qPCR and much more ... Services are provided on non-commercial basis for the EMBL community and academic institutions in the context of scientific collaborations within the EMBL member states. Tight links to industry provide state-of-the-art equipment. The experiments and their results feed directly back into improvements of instruments and technologies, spawning the next generation of scientific questions and technologies.

geNorm VBA applet for Microsoft Excel determines the most stable reference genes from a set of tested genes and calculates a gene expression normalization factor based on the geometric mean of a user-defined number of reference genes.
qBase is a collection of macros for Microsoft Excel
for the management and automated analysis of real-time quantitative PCR data. The program employs a delta-Ct relative quantification model with PCR efficiency correction and multiple reference gene normalization.

Media Partner:


eConferences -- Amplify your knowledge in MDx using qPCR, dPCR and NGS!
This streaming portal is dedicated to scientists from the community of qPCR, digital-PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx).
You’ll find here all the records from more than 420 presentations held at Gene Quantification, qPCR dPCR & NGS and MG Events in the past years – starting qPCR 2010 in Vienna, over MicroGenomics 2014 in Paris, to GQ2019 in Freising-Weihenstephan.
We provide the presentations via streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device.


Technology Networks
Technology Networks was established around 15 years ago with the aim of providing informative websites for life science professionals. The team run, update and provide an outstanding web-based information solution for those working within the Life Science and Drug Discovery community. Since its humble beginnings running just one website based on Combinatorial Chemistry. Today Technology Networks has expanded its portfolio to include over 30 communities: e.g. qPCR, Next Gen Sequencing, AgriGenomics, Molecular & Clinical Diagnostics, Genomics, and Genotyping & Gene Expression is edited by Michael W. Pfaffl
The Gene Quantification web pages are educational and academic sites with the only purpose of facilitating access to biology-related information over the internet.
© 2002-2024     22nd Anniversary    version 192.03  last update  @  8th July 2024    Copyrights    Disclaimer    EU - DSGVO